Pyrosequencing is a method of DNA sequencing that relies on the production of light signal during nucleotide incorporation. It involves the use of enzymes to generate a series of detectable light signals corresponding to the order of nucleotides in a DNA strand. This technique is highly sensitive and accurate, making it a valuable tool for sequencing small DNA fragments, detecting genetic variations, and studying microbial communities. Pyrosequencing has been widely used in genomics, medical research, and microbiology studies.