Cryo-electron microscopy (cryo-EM) is a powerful imaging technique used in structural biology to study the structure of biological molecules and complexes at near-atomic resolution. The samples are rapidly frozen in a thin layer of vitreous ice, preserving their native structure and allowing them to be imaged in their natural state. The technique has revolutionized the field of structural biology by providing detailed insights into the structure and function of a wide range of biomolecules, including proteins, nucleic acids, and viruses. Cryo-EM has become increasingly popular in recent years due to advancements in technology and computational tools, making it a valuable tool for studying complex biological systems.