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Thomas Tomasiak

Assistant Professor, Chemistry and Biochemistry,
Assistant Professor, BIO5 Institute,
Member of the Graduate Faculty,

Chemistry & Biochemistry - Sci
tomasiak@arizona.edu
520/621-4646
UAVitae logogoogle scholar logoOrcid logo
Grant: $1.6M
Our bodies face the tremendous task of sorting, organizing, and safely dealing with the millions of small molecules they encounter every day. These chemicals include nutrients, drugs, and toxins with profound implications for our health and for drug design. Our lab focuses on the main systems that our bodies use to recognize and sort these compounds, especially transporters from the ATP Binding Cassette (ABC) and Solute Carrier Superfamily (SLC) families using a combination of biochemistry, genetics, and structural biology. These transporters have presented challenges owing to their instability once extracted from our cells membranes. The formulation of new lipid isolation systems, new analytical methods, and powerful new structural biology tools, especially powerful new electron microscopes, have cracked these problems open and present an exciting new forefront in understanding fundamental biology as well as in drug design.
Thomas Tomasiak experts in biochemistry, structural biology, biophysics, molecular biology, enzymology, x-ray crystallography, nmr spectroscopy, membrane proteins, cytochrome p450, cryo-em, protein crystallography, bioinformatics, computational biology, crystallography, proteomics, mass spectrometry, medicinal chemistry, systems biology, cell biology, nephrology, microbiology
Courses
Biochemistry 
Proteins and Enzymes 
Biological Structure 1 
Recent Grants
Logo of ATP Binding Cassette (ABC) Transporters in Fungal Drug Tolerance funding agency
2021 $761.1KActive
ATP Binding Cassette (ABC) Transporters in Fungal Drug Tolerance
Logo of Co-Crystallization of CD8ab with Targeting Antibodies funding agency
2021 $30.9KActive
Co-Crystallization of CD8ab with Targeting Antibodies
Logo of Co-Crystallization of CD8ab with Targeting Antibodies funding agency
2021 $15.5KActive
Co-Crystallization of CD8ab with Targeting Antibodies
Logo of Structural mechanisms for ABC multidrug transporter selectivity and transport ABC transporters funding agency
2018 $745.6K
Structural mechanisms for ABC multidrug transporter selectivity and transport ABC transporters
Publications (32)
Recent
2022Asymmetric intermediates in the transport cycle of ABC transporters 
2022Vaccines to Prevent Coccidioidomycosis: A Gene-Deletion Mutant of Coccidioides Posadasii as a Viable Candidate for Human Trials 
2022Biochemical and structural insight of regulation in yeast vacuolar ABC transporter Ycf1 
2022The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation 
2022Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter 
2021The role of novel COQ8B mutations in glomerulopathy and related kidney defects 
2021Cryo-EM structure of an elusive pre-transport intermediate of the multidrug ABC transporter BmrCD reveals modes of asymmetric drug binding 
2021A conserved motif in intracellular loop 1 stabilizes the outward-facing conformation of TmrAB 
2020The peptide sensor motif stabilizes the outward-facing conformation of TmrAB 
2020Structural Insight into Vacuolar ABC Transporter Mlt1 of Candida albicans 
2020Resolving CD47 Structure and Function to Understand Signal Transduction Mechanism 
2020The peptide sensor motifs asymmetrically couple ATP hydrolysis to transport in the heterodimeric ABC transporter TmrAB 
2020Allosteric Coupling in an Asymmetric ABC Transporter 
2018New crystal forms of the integral membrane Escherichia coli quinol: fumarate reductase suggest that ligands control domain movement 
2017Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP 
2017Indole-3-carbinol (I3C) analogues are potent small molecule inhibitors of NEDD4-1 ubiquitin ligase activity that disrupt proliferation of human melanoma cells 
2017Crystal Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP 
2015Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter 
2015Subnanometer resolution cryo-EM structure of a nucleotide free heterodimeric ABC exporter 
2014Transmembrane Architecture of a Full-Length Monospanning Cytochrome P450 
2014’Connell JD, Cannon RD, McDonald JG, Rodriguez A, Finer-Moore JS, Stroud RM. Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the … 
2014Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer 
2014General qPCR and plate reader methods for rapid optimization of membrane protein purification and crystallization using thermostability assays 
2013Plasticity of the quinone-binding site of the complex II homolog quinol: fumarate reductase 
2013Crystallization of Erg11p-the cytochrome P450 target of triazole antifungals 
2012Correction: A Structural Model for Binding of the Serine-Rich Repeat Adhesin GspB to Host Carbohydrate Receptors 
2011A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors 
2011Catalysis, inhibition, and signal transduction by menaquinol: fumarate oxidoreductase 
2011Geometric restraint drives on-and off-pathway catalysis by the Escherichia coli menaquinol: fumarate reductase 
2008A threonine on the active site loop controls transition state formation in Escherichia coli respiratory complex II 
2008S13/2 Conformational changes and activity of complex II 
2007Succinate as donor; fumarate as acceptor 
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