KMap
Our bodies face the tremendous task of sorting, organizing, and safely dealing with the millions of small molecules they encounter every day. These chemicals include nutrients, drugs, and toxins with profound implications for our health and for drug design. Our lab focuses on the main systems that our bodies use to recognize and sort these compounds, especially transporters from the ATP Binding Cassette (ABC) and Solute Carrier Superfamily (SLC) families using a combination of biochemistry, genetics, and structural biology. These transporters have presented challenges owing to their instability once extracted from our cells membranes. The formulation of new lipid isolation systems, new analytical methods, and powerful new structural biology tools, especially powerful new electron microscopes, have cracked these problems open and present an exciting new forefront in understanding fundamental biology as well as in drug design.
Courses
  • BS
    Biological Structure 1

  • PE
    Proteins and Enzymes

  • B
    Biochemistry

Student Thesis/Dissertation
  • The Role of Conserved GRD Motif in the Allosteric Regulation of Ycf1 Transport Activity and Protein Function

    2021

    masters
Grants
  • Funding agency logo
    ATP Binding Cassette (ABC) Transporters in Fungal Drug Tolerance

    Principal Investigator (PI)

    2021

    $1.1M
    Active
  • Funding agency logo
    Co-Crystallization of CD8ab with Targeting Antibodies

    Principal Investigator (PI)

    2021

    $30.9K
    Active
  • Funding agency logo
    Co-Crystallization of CD8ab with Targeting Antibodies

    Principal Investigator (PI)

    2021

    $15.5K
    Active
  • Funding agency logo
    Structural mechanisms for ABC multidrug transporter selectivity and transport ABC transporters

    Principal Investigator (PI)

    2018

    $745.6K
Publications (33)
Recent
  • Structural Basis for Autoinhibition by the Dephosphorylated Regulatory Domain of Ycf1

    2023

  • Asymmetric intermediates in the transport cycle of ABC transporters

    2022

  • Biochemical and structural insight of regulation in yeast vacuolar ABC transporter Ycf1

    2022

  • Vaccines to Prevent Coccidioidomycosis: A Gene-Deletion Mutant of Coccidioides Posadasii as a Viable Candidate for Human Trials

    2022

  • Asymmetric drug binding in an ATP-loaded inward-facing state of an ABC transporter

    2022

  • The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation

    2022

  • Cryo-EM structure of an elusive pre-transport intermediate of the multidrug ABC transporter BmrCD reveals modes of asymmetric drug binding

    2021

  • A Conserved Motif in Intracellular Loo1 Stabilizes the Outward-Facing Conformation of TmrAB

    2021

  • Resolving CD47 Structure and Function to Understand Signal Transduction Mechanism

    2020

  • Structural Insight into Vacuolar ABC Transporter Mlt1 of Candida albicans

    2020

  • The peptide sensor motifs asymmetrically couple ATP hydrolysis to transport in the heterodimeric ABC transporter TmrAB

    2020

  • Allosteric Coupling in an Asymmetric ABC Transporter

    2020

  • The peptide sensor motif stabilizes the outward-facing conformation of TmrAB

    2020

  • New crystal forms of the integral membrane Escherichia coli quinol: fumarate reductase suggest that ligands control domain movement

    2018

  • Indole-3-carbinol I3C) analogues are potent small molecule inhibitors of NEDD4-1 ubiquitin ligase activity that disrupt proliferation of human melanoma cells

    2017

  • Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP

    2017

  • Crystal Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP

    2017

  • Subnanometer resolution cryo-EM structure of a nucleotide free heterodimeric ABC exporter

    2015

  • Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter

    2015

  • Transmembrane Architecture of a Full-Length Monospanning Cytochrome P450

    2014

  • General qPCR and plate reader methods for rapid optimization of membrane protein purification and crystallization using thermostability assays

    2014

  • Architecture of a single membranening cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer

    2014

  • ’Connell JD, Cannon RD, McDonald JG, Rodriguez A, Finer-Moore JS, Stroud RM. Architecture of a single membranening cytochrome P450 suggests constraints that orient the …

    2014

  • Crystallization of Erg11p-the cytochrome P450 target of triazole antifungals

    2013

  • Plasticity of the quinone-binding site of the complex II homolog quinol: fumarate reductase

    2013

  • Correction: A Structural Model for Binding of the Serine-Rich Repeat Adhesin GspB to Host Carbohydrate Receptors

    2012

  • Geometric restraint drives on-and off-pathway catalysis by the Escherichia coli menaquinol: fumarate reductase

    2011

  • A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors

    2011

  • Catalysis, inhibition, and signal transduction by menaquinol: fumarate oxidoreductase

    2011

  • S13/2 Conformational changes and activity of complex II

    2008

  • A threonine on the active site loocontrols transition state formation in Escherichia coli respiratory complex II

    2008

  • Succinate as donor; fumarate as acceptor

    2007

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