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Roger L. Miesfeld, Ph.D., Professor and Co-Chair, Dept. of Chemistry & Biochemistry, College of Science, University of Arizona. Mosquitoes are human disease vectors that transmit pathogens through blood feeding. One of these disease vectors is the Aedes aegypti mosquito, which have rapidly expanded their habitat and are contributing annually to 500,000 cases of Dengue hemorrhagic fever. On an even greater scale, Anopheline mosquitoes account for 250 million cases of malaria/yr, with up to 1 million deaths annually. The most common adult insecticides used for mosquito control are pyrethroids, which inhibit evolutionarily conserved sodium channels in the mosquito nervous system. Although these compounds have proven to be effective, mosquito resistance is an increasing problem and there is a pressing need to develop the next generation of safe and effective agents. Since blood meal feeding creates a unique metabolic challenge as a result of the extremely high protein and iron content of blood, it is possible that interfering with blood meal metabolism could provide a novel control strategy for mosquito born diseases. Our long term goal is to identify small molecule inhibitors that block blood meal metabolism in vector mosquitoes, resulting in feeding-induced death of the adult female, or a significant reduction in egg viability, as a strategy to control vector mosquito populations in areas of high disease transmission.
Courses
  • B
    Biochemistry

  • IBR
    Introduction to Biochemical Research

  • FB
    Foundations in Biochemistry

  • MB
    Metabolic Biochemistry

  • GPG
    General Protein and General Metabolic Biochemistry

Student Thesis/Dissertation
  • PROBING ANOPHELES STEPHENSI MOSQUITO MIDGUT WITH PROTECTED TRIAZABUTADIENES

    2022

    bachelors
  • INVESTIGATING THE STRUCTURAL CHARACTERISTICS OF AN hMC4R SELECTIVE AGONIST

    2021

    bachelors
  • Linking Culture Chemistry To Isolation Success And Microbial Inhibition Potential Of Microbial Symbionts From Laurus Nobilis

    2019

  • Validation of a Semi-Automatic Cell Segmentation Method to the Manual Cell Counting Method on Identifying Proliferating Cells in 3-D Confocal Microscope Images

    2018

    bachelors
  • Molecular and Biochemical Characterization of Chorion Peroxidases During Eggshell Formation in Aedes aegypti

    2017

    bachelors
  • Identification of Regulators of Lysosome Formation

    2017

    bachelors
  • ANALYSIS OF MYOGENIC MARKERS IN DUCHENNE MUSCULAR DYSTROPHY CELL MODELS

    2016

    Bachelors
  • An Investigation of Cellular Proliferation and Nuclear Morphology in the Multi-Step Progression of Cutaneous Squamous Cell Carcinoma

    2015

    bachelors
Grants
  • Funding agency logo
    Graduate Student Internships at Luceome Biotechnologies

    Principal Investigator (PI)

    2011

    $718.0K
  • Funding agency logo
    Regulation of Digestion in Blood-Sucking Insects

    Principal Investigator (PI)

    2006

    $1.6M
  • Funding agency logo
    Regulation of Energy Metabolism in Insects

    Principal Investigator (PI)

    2006

    $1.6M
Technologies / Patents
      Books
      • Biochemistry, 2nd Edition

        2021

      • Biochemistry, 1st Edition

        2017

      • Applied Molecular Genetics

        1999

      • Biochemistry: A Short Course

        1997

      News
      • Outstanding Graduates Will Be Honored for Service, Academics and Perseverance

        2021

      • Fighting Human Disease with Birth Control… for Mosquitoes

        2019

      • I-Squared Awards Honor Inventors, Entrepreneurs, Ecosystem Champions

        2018

      • UA Online: All This, and Science Too

        2017

      • Making Blood-Sucking Deadly for Mosquitoes

        2011

      • Researchers Put the Bite on Mosquitoes

        2008

      • The Force Behind 'WaveLengths'

        2008

      • Arizona Public Media Launches New Series

        2008

      • Faculty Senate Election Leaves Seats Unfilled

        2002

      Publications (63)
      Recent
      • Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes

        2019

      • COPI-mediated blood meal digestion in vector mosquitoes is independent of midgut ARF-GEF and ARF-GAP regulatory activities

        2013

      • Distinct biological effects of golgicide a derivatives on larval and adult mosquitoes

        2012

      • Defects in coatomer protein I COPI) transport cause blood feeding-induced mortality in Yellow Fever mosquitoes

        2011

      • Alpha-COPI coatomer protein is required for rough endoplasmic reticulum whorl formation in mosquito midgut epithelial cells

        2011

      • Deficiencies in acetyl-CoA carboxylase and fatty acid synthase 1 differentially affect eggshell formation and blood meal digestion in Aedes aegypti

        2011

      • In vitro activation and enzyme kinetic analysis of recombinant midgut serine proteases from the Dengue vector mosquito Aedes aegypti

        2011

      • Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti

        2010

      • Differentiation and quantification of C1 and C2 13)C-labeled glucose by tandem mass spectrometry

        2010

      • Differential ammonia metabolism in Aedes aegypti fat body and midgut tissues

        2010

      • Energy metabolism during diapause in Culex pipiens mosquitoes

        2009

      • Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes

        2009

      • Molecular analysis of the Aedes aegypti carboxypeptidase gene family

        2009

      • Discovery of an alternate metabolic pathway for urea synthesis in adult Aedes aegypti mosquitoes

        2008

      • TOR signaling is required for amino acid stimulation of early trypsin protein synthesis in the midgut of Aedes aegypti mosquitoes

        2008

      • Regulated expression of microinjected DNA in adult Aedes aegypti mosquitoes

        2007

      • Cloning and characterization of a novel MyoD enhancer-binding factor

        2007

      • Increased expression of the metastasis-associated gene Ehm2 in prostate cancer

        2006

      • Expression of the cochaperone HspBP1 is not coordinately regulated with Hsp70 expression

        2006

      • Androgen control of cell proliferation and cytoskeletal reorganization in human fibrosarcoma cells: role of RhoB signaling

        2004

      • Differential control of eosinophil survival by glucocorticoids

        2004

      • Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation

        2003

      • Identification of a Novel Glucocorticoid Receptor Mutation in Budesonide-Resistant Human Bronchial Epithelial Cells

        2003

      • Glucocorticoid regulation of human eosinophil gene expression

        2003

      • Androgen induction of in vitro prostate cell differentiation

        2002

      • Analysis of glucocorticoid and androgen receptor gene fusions delineates domains required for transcriptional specificity

        2001

      • Characterization of Apt- cell lines exhibiting cross-resistance to glucocorticoid- and Fas-mediated apoptosis

        1999

      • Glucocorticoid regulation of GM-CSF: evidence for transcriptional mechanisms in airway epithelial cells

        1998

      • Crosstalk during Ca2+ cAMP- and glucocorticoid-induced gene expression in lymphocytes

        1997

      • Glucocorticoid receptor signaling in a bronchial epithelial cell line

        1997

      • Transcriptional control of steroid-regulated apoptosis in murine thymoma cells

        1996

      • Delineation of two distinct type 1 activation functions in the androgen receptor amino-terminal domain

        1996

      • Adenomatous polyposis coli, protein kinases, protein tyrosine phosphatase: the effect of sulindac

        1995

      • Isolation of differentially expressed sequence tags from steroid-responsive cells using mRNA differential display

        1995

      • A cell-specific and selective effect on transactivation by the androgen receptor

        1995

      • Quantitative differences in androgen and glucocorticoid receptor DNA binding properties contribute to receptor-selective transcriptional regulation

        1995

      • Evidence that BCL-2 represses apoptosis by regulating endoplasmic reticulum-associated Ca su2+ su fluxes

        1994

      • The length and location of CAG trinucleotide repeats in the androgen receptor N-terminal domain affect transactivation function

        1994

      • Recessive mutations in a common pathway block thymocyte apoptosis induced by multiple signals

        1994

      • Elevated glutathione S-transferase gene expression is an early event during steroid-induced lymphocyte apoptosis

        1993

      • Evidence that glucocorticoid- and cyclic AMP-induced apoptotic pathways in lymphocytes share distal events

        1992

      • Characterization of a panel of rat ventral prostate epithelial cell lines immortalized in the presence or absence of androgens

        1992

      • Transcriptional transactivation functions localized to the glucocorticoid receptor N terminus are necessary for steroid induction of lymphocyte apoptosis

        1992

      • Stable expression of the calbindin-D28K complementary DNA interferes with the apoptotic pathway in lymphocytes

        1992

      • Isolation and characterization of transcripts induced by androgen withdrawal and apoptotic cell death in the rat ventral prostate

        1991

      • Transcriptional analyses of steroid-regulated gene networks

        1990

      • nti glucocorticoid receptor transcripts lack sequences encoding the amino-terminal transcriptional modulatory domain

        1990

      • Functional characterizations of the androgen receptor confirm that the molecular basis of androgen action is transcriptional regulation

        1990

      • Molecular genetics of corticosteroid action

        1990

      • The structure and function of steroid receptor proteins

        1989

      • Mechanism of glucocorticoid receptor down-regulation by glucocorticoids

        1988

      • Glucocorticoid receptor mutants that define a small region sufficient for enhancer activation

        1987

      • Intracellular receptor concentration limits glucocorticoid-dependent enhancer activity.

        1987

      • Characterization of glucocorticoid receptors and glucocorticoid receptor mRNA in human leukemia cells: Stabilization of the receptor by diisopropylfluorophosphate

        1987

      • Glucocorticoid receptor mutants that are constitutive activators of transcriptional enhancement

        1987

      • Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA

        1986

      • Species-specific rDNA transcription is due to promoter-specific binding factors

        1984

      • Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells

        1984

      • The absence of a human-specific ribosomal DNA transcription factor leads to nucleolar dominance in mouse human hybrid cells

        1984

      • Identification of the in vivo and in vitro origin of transcription in human rDNA

        1982

      • A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human δ and β globin genes

        1981

      • Studies on the nature and germ-line stability of DNA sequences flanking the mouse immunoglobulin heavy-chain constant-region genes

        1981

      • Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome

        1980

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