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William Montfort received BS and PhD degrees in Chemistry (Biochemistry emphasis) from Oakland University (1980) and the University of Texas (1985). His graduate studies, with Prof. Jon Robertus, led to the crystal structure of ricin. In his postdoctoral studies, with Prof. Robert Stroud at the University of California, San Francisco, he determined crystal structures of anticancer drug target thymidylate synthase. He moved to the University of Arizona in 1989, where he established a laboratory focused on protein structure/function and founded the X-ray crystallography facility. He is now Professor of Chemistry and Biochemistry, Director of the NIH-funded Biological Chemistry Graduate Program and Co-Lead for Clinical and Translational Oncology Program in the Cancer Center.Show Less
Courses
- BBiochemistry
- NAMNucleic Acids, Metabolism, and Signaling
- BSIBiological Structure I
- BSBiological Structure 1
- MBMetabolic Biochemistry
Student Thesis/Dissertation
- Structural and Biochemical Studies of DYRK1A Kinase
2023
doctoral - Developing a screen to discover novel mRNA 3’-UTR-mediated scaffolding of protein- protein interactors
2022
bachelors - Functional Expression of Organic Cation Transporters at the Blood-Brain Barrier: Relevance to the Treatment of Ischemic Stroke
2022
bachelors - MECHANISMS UNDERLYING THE PROTECTIVE EFFECT OF GLYCOLYSIS
2022
bachelors - Determining CD47 Structure and Discerning Function to Understand Signal Transduction Mechanism
2021
doctoral - Allosteric Regulation of Soluble Guanylyl Cyclase: The Nitric Oxide Receptor
2018
doctoral - X-Ray Crystallographic Studies of Dyrk1a, a Kinase Linked to Down Syndrome
2017
bachelors - Uncovering Signal Transduction in Blood Pressure Regulation by Nitric Oxide
2017
bachelors - Mass Spectrometry: Toward Elucidating the Biosignature of Coccidioidomycosis and Insights into Surface Induced Dissociation of Biologically Relevant Carbohydrates
2016
doctoral - Angiotensin-(1-7) as an Antinociceptive Agent in Cancer-Induced Bone Pain
2015
bachelors - Structural Studies of Thioredoxin S-nitrosation and Detection of Protein S-nitrosothiols by Phosphine Derivatization
2014
doctoral - The Mechanism of Allosteric Regulation in Soluble Guanylate Cyclase
2014
doctoral
Grants
- A Nutrition and Exercise Prehabilitation Intervention on Inflammatory Biomarkers in American Indian Cancer Patients
Key Personnel (KP)
2023
$271.6KActive - Establishing a Pleiotropic Brain-Penetrant Small-Molecule to Impede Glioblastoma
Co-Investigator (COI)
2023
$180.7KActive - DYRK1A Inhibition as a Novel Treatment Approach for Alzheimer's Disease
Co-Investigator (COI)
2020
$3.0MActive - Chemistry-Biology Interface Training Program
Principal Investigator (PI)
2019
$1.5MActive - Addressing Breast Cancer Health Disparities in American Indians through Translational and Dissemination/Implementation Science (Diversity Supplement for Dr. Jennifer Erdrich - Cost Extension)
Key Personnel (KP)
2019
$346.2KActive - Structure and Stimulator Binding Mechanism of the Nitric Oxide Receptor, Soluble Guanylate Cyclase
Key Personnel (KP)
2017
$106.8K - University of Arizona Cancer Center - Cancer Center Support Grant
Key Personnel (KP)
2016
$26.3M - Soluble Guanylate Cyclase: A Therapeutic Target for Cardiovascular Pathology
Co-Investigator (COI)
2016
$50.3K - Nitric Oxide Signaling in Health and Disease
Principal Investigator (PI)
2015
$1.4M - Chemistry-Biology Interface Training Program
Principal Investigator (PI)
2014
$1.1M
News
- Researchers Paving the Way for New Heart Disease Treatments
2008
- Spotlight on Teaching: Wells Gives Students 3-D Understanding of Molecules
2000
Publications (136)
Recent
- Sorting nexin-dependent therapeutic targeting of oncogenic epidermal growth factor receptor
2023
- Abstract P5-10-08: Inflammation drives NOS2-Akt2 signaling in triple negative breast cancer
2022
- Solution structures of the Shewanella woodyi H‐NOX protein in the presence and absence of soluble guanylyl cyclase stimulator IWP‐051
2021
- Resolving CD47 Structure and Function to Understand Signal Transduction Mechanism
2020
- SOLUTION STRUCTURE OF THE IWP-051-BOUND H-NOX FROM SHEWANELLA WOODYI REVEALS A CONSERVED BINDING POCKET FOR SOLUBLE GUANYLYL CYCLASE STIMULATORS
2019
- Instability in a coiled‐coil signaling helix is conserved for signal transduction in soluble guanylyl cyclase
2019
- Method for detecting crosslinked peptides via reproducible fragmentation in a mass spectrometer
2019
- Underlying molecular alterations in human dihydrolipoamide dehydrogenase deficiency revealed by structural analyses of disease-causing enzyme variants
2019
- Unloading the left ventricle before reperfusion in patients with anterior ST-segment–elevation myocardial infarction: a pilot study using the Impella CP
2019
- Detectable IgG against a Cimex lectularius allergen after a report of bed bug bites among NYC children
2019
- How stimulator compounds and nitric oxide bind and stimulate soluble guanylyl cyclase GC1)
2019
- Novel nitric oxide-liberating heme proteins from the saliva of bloodsucking insects
2018
- Discovery of stimulator binding to a conserved pocket in the heme domain of soluble guanylyl cyclase
2018
- MetaUniDec: high-throughput deconvolution of native mass spectra
2018
- Blocking “don't eat me” signal of CD47-SIRPα in hematological malignancies, an in-depth review
2018
- Characterizing the CD47/TSP-1 Signaling Axis
2018
- A sporadic case of severe peripheral neuropathy expands the phenotypic range of TUBB2A mutations.
2017
- Structure and Activation of Soluble Guanylyl Cyclase, the Nitric Oxide Sensor
2016
- Localizing Stimulator Binding to the beta 1 H-NOX Domain of Soluble Guanylate Cyclase
2016
- Determining Nitric Oxide‐induced Conformational Changes in Soluble Guanylate Cyclase using Lanthanide‐based Resonance Energy Transfer
2016
- Uncovering Stimulator Binding Mechanism to Soluble Guanylyl Cyclase by Solution NMR
2016
- Heat shock protein 90 associates with the Per-Arnt-Sim domain of heme-free soluble guanylate cyclase: implications for enzyme maturation
2015
- Dimerization of nitrophorin 4 at low pH and comparison to the K1A mutant of nitrophorin 1
2015
- Development of IgE against a Cimex lectularius allergen after being bitten by bed bugs was common among children in NYC
2014
- YC-1 binding to the β subunit of soluble guanylyl cyclase overcomes allosteric inhibition by the α subunit
2014
- Evolution in an ancient detoxification pathway is coupled with a transition to herbivory in the drosophilidae
2014
- Molecular model of a soluble guanylyl cyclase fragment determined by small-angle X-ray scattering and chemical cross-linking
2013
- YC‑1 Binding to the β Subunit of Soluble Guanylyl Cyclase Overcomes Allosteric Inhibition by the α Subunit
2013
- pH-dependent picosecond structural dynamics in the distal pocket of nitrophorin 4 investigated by 2D IR spectroscopy
2013
- Structural Studies of Thioredoxin S-nitrosation and Detection of Protein S-nitrosothiols by Phosphine Derivatization
2013
- Crystal structure of the Alpha subunit PAS domain from soluble guanylyl cyclase
2013
- Rational reprogramming of fungal polyketide first-ring cyclization
2013
- Nitric Oxide Signaling with Heme Proteins
2012
- Structural and Functional Studies on the Nitric Oxide Receptor: YC-1 Binding to the PAS Domain of Soluble Guanylate Cyclase
2012
- Thrombospondin-1 Binding to CD47 in the Regulation of Nitric Oxide Signaling
2012
- IgE against bed bug Cimex lectularius) allergens is common among adults bitten by bed bugs
2012
- Distal pocket and belt side chain effects on the stabilization of the ferric-NO heme center of the Nitrophorins of Rhodnius prolixus
2011
- Oxidation and Loss of Heme in Soluble Guanylyl Cyclase from Manduca sexta
2011
- Crystal structures of multicopper oxidase CueO bound to copper I) and silver I) functional role of a methionine-rich sequence
2011
- Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration
2011
- The Reaction of HNO Donors with Heme Proteins
2010
- Activation mechanism of soluble guanylate cyclase: New insights from the resonance Raman studies of a truncated insect protein
2010
- Model for Truncated Soluble Guanylate Cyclase by Negative Stain Electron Microscopy: 384
2010
- Ultrafast dynamics of diatomic ligand binding to nitrophorin 4
2010
- Investigating shape and mechanism of soluble guanylate cyclase: 383
2010
- Crystal structure of human thioredoxin revealing an unraveled helix and exposed S‐nitrosation site
2010
- NM23-H2 may play an indirect role in transcriptional activation of c-myc gene expression but does not cleave the nuclease hypersensitive element III1NM23-H2 and c-Myc Activation
2009
- Crystal structure of the bacterial luciferase/flavin complex provides insight into the function of the β subunit
2009
- NITRIC OXIDE SIGNALING IN BLOOD‐SUCKING INSECTS, HORNWORMS AND HUMANS
2009
- Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and …
2009
- A New Use for a Familiar Fold: The X-ray Crystal Structure of GTP-Bound GTP Cyclohydrolase III from Methanocaldococcus jannaschii Reveals a Two Metal Ion Catalytic …
2008
- N15 Cro and λ Cro: Orthologous DNA‐binding domains with completely different but equally effective homodimer interfaces
2008
- Allostery in recombinant soluble guanylyl cyclase from Manduca sexta
2008
- Low-frequency mode activity of heme: femtosecond coherence spectroscopy of iron porphine halides and nitrophorin
2008
- Transitive homology-guided structural studies lead to discovery of Cro proteins with 40% sequence identity but different folds
2008
- Binding of YC-1 or BAY 41-2272 to soluble guanylyl cyclase induces a geminate phase in CO photolysis
2008
- Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: calorimetric and crystallographic analysis of the K48Q mutant
2008
- X-ray Structure of Cerulean GFP: A Tryptophan-Based Chromophore Useful for Fluorescence Lifetime Imaging,
2007
- Apo‐nitrophorin 4 at atomic resolution
2007
- Buried S-Nitrosocysteine Revealed in Crystal Structures of Human Thioredoxin,
2007
- S-nitrosylation-induced conformational change in blackfin tuna myoglobin
2007
- Nonequilibrium dynamics simulations of nitric oxide release: comparative study of nitrophorin and myoglobin
2007
- Assignment of the Ferriheme Resonances of the Low-Spin Complexes of Nitrophorins 1 and 4 by 1H and 13C NMR Spectroscopy: Comparison to Structural Data …
2007
- Microsystems for UV-visible and x-ray analysis of protein crystals
2007
- O19. Crystal structures of S-nitrosated thioredoxin
2006
- Structural characterization of R. prolixus nitrophorin 4 by UV and visible resonance Raman spectroscopy
2006
- O11. HNO donors and heme proteins
2006
- Crystal structures of thymidylate synthase mutant R166Q: structural basis for the nearly complete loss of catalytic activity
2006
- SYMPOSIUM LECTURES-Nitrosyl heme and S-nitrosation in nitric oxide signaling
2006
- Structure of the Y94F mutant of Escherichia coli thymidylate synthase
2006
- P062. NO and GSNO stimulation of recombinant soluble guanylate cyclase from Manduca sexta
2006
- A Novel Copper-Binding Fold for the Periplasmic Copper Resistance Protein CusF,
2005
- 5, 10-Methylene-5, 6, 7, 8-tetrahydrofolate conformational transitions upon binding to thymidylate synthase: molecular mechanics and continuum solvent studies
2005
- Ultrahigh Resolution Structures of Nitrophorin 4: Heme Distortion in Ferrous CO and NO Complexes,
2005
- Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein
2005
- Loodynamics and function in nitrophorin 4
2004
- Resonance Raman spectroscopic study of nitrophorin 2 from Rhodnius prolixus kissing bug and its mutants
2004
- Cuprous Oxidase Activity of CueO from Escherichia coli
2004
- Protein and ligand induced heme distortion in the nitrophorins
2004
- Role of Binding Site Loops in Controlling Nitric Oxide Release: Structure and Kinetics of Mutant Forms of Nitrophorin 4,
2004
- Axial ligand complexes of the Rhodnius nitrophorins: reduction potentials, binding constants, EPR spectra, and structures of the 4-iodopyrazole and imidazole …
2004
- Structural dynamics controls nitric oxide affinity in nitrophorin 4
2004
- HEME DEFORMATION AND A NOVEL HEME-ASSISTED NITROSILATION OF A THIOLATE LIGAND IN NITROPHORINS FROM BLOOD-SUCKING INSECTS
2004
- Heme ruffling in nitrophorin 4, a NO transport protein from Rhodnius prolixus
2004
- Protein Functional Cycle Viewed at Atomic Resolution: Conformational Change and Mobility in Nitrophorin 4 as a Function of pH and NO Binding,
2004
- Cimex lectularius nitrophorin characterized by resonance Raman spectroscopy
2004
- Nitric oxide transport by blood-sucking insects: Structure and dynamics
2003
- A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO
2003
- Complementing thymidylate synthase
2003
- Redox potential of human thioredoxin 1 and identification of a second dithiol/disulfide motif
2003
- Nitrophorins: Lipocalin‐based heme proteins transporting nitric oxide
2002
- Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli
2002
- Blocking ligand escape with a stochastic gate in nitrophorin-NO complex
2002
- INSECT Rhodnius prolixus
2001
- Spectroelectrochemical studies of nitrophorin mutants: The importance of the carboxylates near the heme.
2001
- Ligand-Induced Heme Ruffling and Bent NO Geometry in Ultra-High-Resolution Structures of Nitrophorin 4,
2001
- Resonance Raman Spectroscopic Study of Nitrophorin 1, a Nitric Oxide-Binding Heme Protein from Rhodnius prolixus, and Its Nitrosyl and Cyano Adducts
2001
- Properties and biological activities of thioredoxins
2001
- Nitric oxide transport by a blood-sucking insect: Nitrophorin structure, dynamics, and distal pocket burial
2000
- Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial
2000
- The crystal structure of Nitrophorin 2: A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus
2000
- Kinetics and equilibria in ligand binding by nitrophorins 1− 4: Evidence for stabilization of a nitric oxide− ferriheme complex through a ligand-induced conformational trap
2000
- The nitric oxide-releasing heme proteins from the saliva of the blood-sucking insect Rhodnius prolixus
2000
- α-Cyclodextrin extracts diacylglycerol from insect high density lipoproteins
2000
- THE NITRIC OXIDE-RELEASING HEME PROTEINS
2000
- Structure and reactivity of nitrophorins from a blood-sucking insect
1999
- Structural, spectroscopic and electrochemical comparison of three of the four nitrophorins NO-releasing heme proteins) from the saliva of the blood-sucking insect, Rhodnius …
1999
- In vivo inhibition of histamine-induced vascular leaks with kissing bug R prolixus) salivary protein NP4.
1999
- Nitric oxide binding to the ferri-and ferroheme states of nitrophorin 1, a reversible NO-binding heme protein from the saliva of the blood-sucking insect, Rhodnius prolixus
1999
- Crystal Structures of Rat Thymidylate Synthase Inhibited by Tomudex, a Potent Anticancer Drug,
1999
- Mode of action of site-directed irreversible folate analogue inhibitors of thymidylate synthase
1998
- Crystal structures of a nitric oxide transport protein from a blood-sucking insect
1998
- The crystal structure of nitrophorin 4 at 1.5 Å resolution: transport of nitric oxide by a lipocalin-based heme protein
1998
- Human Thioredoxin Homodimers: Regulation by pH, Role of Aspartate 60, and Crystal Structure of the Aspartate 60 → Asparagine Mutant,
1997
- Use of strain in a stereospecific catalytic mechanism: crystal structures of Escherichia coli thymidylate synthase bound to FdUMP and methylenetetrahydrofolate
1997
- Reversible no-carrying heme proteins from the saliva of bloodsucking insects.
1997
- Nitric Oxide Binding and Crystallization of Recombinant Nitrophorin I, a Nitric Oxide Transport Protein from the Blood-Sucking Bug Rhodnius prolixus
1997
- Thymidylate synthase: structure, inhibition, and strained conformations during catalysis
1997
- Crystal structures of a marginally active thymidylate synthase mutant, Arg 126→ Glu
1997
- Oxidative inactivation of thioredoxin as a cellular growth factor and protection by a Cys73→ Ser mutation
1996
- Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer
1996
- Promotion of purine nucleotide binding to thymidylate synthase by a potent folate analogue inhibitor, 1843U89.
1995
- Ligand-induced distortion of an active site in thymidylate synthase upon binding anticancerdrug 1843U89
1995
- Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase …
1995
- Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex
1994
- Binding by Thymidylate Synthase1
1990
- Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate
1990
- Pairwise specificity and sequential binding in enzyme catalysis: thymidylate synthase
1990
- Segmental accommodation: A novel conformational change induced upon ligand binding by thymidylate synthase
1990
- Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases
1990
- The three-dimensional structure of ricin at 2.8 A.
1987
- Atomic structure of thymidylate synthase: target for rational drug design
1987
- X-ray crystallographic studies on the toxic protein ricin
1985
- Coordination chemistry of alkali and alkaline earth cations: Synthesis and X-ray crystal structure of cesium picrate)benzo-15-crown-5) Cs+ C 6 H 2 N 3 O 7−(C 14 H 20 O 5)
1985
- THE X-RAY STRUCTURE OF RICIN AND IMPLICATIONS FOR IMMUNOTOXIN DESIGN
1984
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