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William Montfort

Professor, Applied Mathematics - GIDP,

Member of the Graduate Faculty,

Professor, Chemistry and Biochemistry - Med,

Professor, Genetics - GIDP,

Professor, Chemistry and Biochemistry-Sci,

Professor, Cancer Biology - GIDP,

Professor, Molecular and Cellular Biology,

Professor, BIO5 Institute,

Chemistry & Biochemistry - Sci
montfort@arizona.edu

520/621-1884

Grant: $43.6M

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William Montfort received BS and PhD degrees in Chemistry (Biochemistry emphasis) from Oakland University (1980) and the University of Texas (1985). His graduate studies, with Prof. Jon Robertus, led to the crystal structure of ricin. In his postdoctoral studies, with Prof. Robert Stroud at the University of California, San Francisco, he determined crystal structures of anticancer drug target thymidylate synthase. He moved to the University of Arizona in 1989, where he established a laboratory focused on protein structure/function and founded the X-ray crystallography facility. He is now Professor of Chemistry and Biochemistry, Director of the NIH-funded Biological Chemistry Graduate Program and Co-Lead for Clinical and Translational Oncology Program in the Cancer Center.

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Courses

Biochemistry

Nucleic Acids, Metabolism, and Signaling

Metabolic Biochemistry

Biological Structure I

Biological Structure 1

Recent Grants

	2020	$2.3M	Active
DYRK1A Inhibition as a Novel Treatment Approach for Alzheimer's Disease
	2019	$1.2M	Active
Chemistry-Biology Interface Training Program
	2019	$346.2K	Active
Addressing Breast Cancer Health Disparities in American Indians through Translational and Dissemination/Implementation Science (Diversity Supplement for Dr. Jennifer Erdrich - Cost Extension)
	2017	$106.8K
Structure and Stimulator Binding Mechanism of the Nitric Oxide Receptor, Soluble Guanylate Cyclase
	2016	$26.3M
University of Arizona Cancer Center - Cancer Center Support Grant
	2016	$50.3K
Soluble Guanylate Cyclase: A Therapeutic Target for Cardiovascular Pathology
	2015	$1.4M
Nitric Oxide Signaling in Health and Disease
	2014	$1.1M
Chemistry-Biology Interface Training Program
	2014	$255.2K
Evaluation of Soluble Guanylyl Cyclase Stimulating Compounds
	2014	$140.0K
YC-1 Stimulation of Soluble Guanylyl Cyclase

News

2008	Researchers Paving the Way for New Heart Disease Treatments
2000	Spotlight on Teaching: Wells Gives Students 3-D Understanding of Molecules

Publications (139)

Recent

2022	Abstract P5-10-08: Inflammation drives NOS2-Akt2 signaling in triple negative breast cancer
2022	Sorting nexin-dependent therapeutic targeting of oncogenic epidermal growth factor receptor
2021	Solution structures of the Shewanella woodyi H‐NOX protein in the presence and absence of soluble guanylyl cyclase stimulator IWP‐051
2020	Resolving CD47 Structure and Function to Understand Signal Transduction Mechanism
2019	SOLUTION STRUCTURE OF THE IWP-051-BOUND H-NOX FROM SHEWANELLA WOODYI REVEALS A CONSERVED BINDING POCKET FOR SOLUBLE GUANYLYL CYCLASE STIMULATORS
2019	Method for detecting crosslinked peptides via reproducible fragmentation in a mass spectrometer
2019	Unloading the left ventricle before reperfusion in patients with anterior ST-segment–elevation myocardial infarction: a pilot study using the Impella CP
2019	How stimulator compounds and nitric oxide bind and stimulate soluble guanylyl cyclase (GC1)
2019	Underlying molecular alterations in human dihydrolipoamide dehydrogenase deficiency revealed by structural analyses of disease-causing enzyme variants
2019	Instability in a coiled‐coil signaling helix is conserved for signal transduction in soluble guanylyl cyclase
2019	Detectable IgG against a Cimex lectularius allergen after a report of bed bug bites among NYC children
2018	Novel nitric oxide-liberating heme proteins from the saliva of bloodsucking insects
2018	Characterizing the CD47/TSP-1 Signaling Axis
2018	MetaUniDec: high-throughput deconvolution of native mass spectra
2018	Blocking “don't eat me” signal of CD47-SIRPα in hematological malignancies, an in-depth review
2018	Discovery of stimulator binding to a conserved pocket in the heme domain of soluble guanylyl cyclase
2017	A sporadic case of severe peripheral neuropathy expands the phenotypic range of TUBB2A mutations.
2016	Localizing Stimulator Binding to the beta 1 H-NOX Domain of Soluble Guanylate Cyclase
2016	Structure and Activation of Soluble Guanylyl Cyclase, the Nitric Oxide Sensor
2016	Uncovering Stimulator Binding Mechanism to Soluble Guanylyl Cyclase by Solution NMR
2016	Determining Nitric Oxide‐induced Conformational Changes in Soluble Guanylate Cyclase using Lanthanide‐based Resonance Energy Transfer
2015	Dimerization of nitrophorin 4 at low pH and comparison to the K1A mutant of nitrophorin 1
2015	Heat shock protein 90 associates with the Per-Arnt-Sim domain of heme-free soluble guanylate cyclase: implications for enzyme maturation
2014	YC-1 binding to the β subunit of soluble guanylyl cyclase overcomes allosteric inhibition by the α subunit
2014	Development of IgE against a Cimex lectularius allergen after being bitten by bed bugs was common among children in NYC
2014	Evolution in an ancient detoxification pathway is coupled with a transition to herbivory in the drosophilidae
2013	Rational reprogramming of fungal polyketide first-ring cyclization
2013	Structural Studies of Thioredoxin S-nitrosation and Detection of Protein S-nitrosothiols by Phosphine Derivatization
2013	pH-dependent picosecond structural dynamics in the distal pocket of nitrophorin 4 investigated by 2D IR spectroscopy
2013	Crystal structure of the Alpha subunit PAS domain from soluble guanylyl cyclase
2013	Molecular model of a soluble guanylyl cyclase fragment determined by small-angle X-ray scattering and chemical cross-linking
2013	YC‑1 Binding to the β Subunit of Soluble Guanylyl Cyclase Overcomes Allosteric Inhibition by the α Subunit
2012	Nitric Oxide Signaling with Heme Proteins
2012	Thrombospondin-1 Binding to CD47 in the Regulation of Nitric Oxide Signaling
2012	Structural and Functional Studies on the Nitric Oxide Receptor: YC-1 Binding to the PAS Domain of Soluble Guanylate Cyclase
2012	IgE against bed bug (Cimex lectularius) allergens is common among adults bitten by bed bugs
2011	Distal pocket and belt side chain effects on the stabilization of the ferric-NO heme center of the Nitrophorins of Rhodnius prolixus
2011	Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration
2011	Crystal structures of multicopper oxidase CueO bound to copper (I) and silver (I): functional role of a methionine-rich sequence
2011	Oxidation and Loss of Heme in Soluble Guanylyl Cyclase from Manduca sexta
2010	The Reaction of HNO Donors with Heme Proteins
2010	Investigating shape and mechanism of soluble guanylate cyclase: 383
2010	Model for Truncated Soluble Guanylate Cyclase by Negative Stain Electron Microscopy: 384
2010	Crystal structure of human thioredoxin revealing an unraveled helix and exposed S‐nitrosation site
2010	Activation mechanism of soluble guanylate cyclase: New insights from the resonance Raman studies of a truncated insect protein
2010	Ultrafast dynamics of diatomic ligand binding to nitrophorin 4
2009	Crystal structure of the bacterial luciferase/flavin complex provides insight into the function of the β subunit
2009	NM23-H2 may play an indirect role in transcriptional activation of c-myc gene expression but does not cleave the nuclease hypersensitive element III1NM23-H2 and c-Myc Activation
2009	NITRIC OXIDE SIGNALING IN BLOOD‐SUCKING INSECTS, HORNWORMS AND HUMANS
2009	Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and …
2008	Transitive homology-guided structural studies lead to discovery of Cro proteins with 40% sequence identity but different folds
2008	Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: calorimetric and crystallographic analysis of the K48Q mutant
2008	A New Use for a Familiar Fold: The X-ray Crystal Structure of GTP-Bound GTP Cyclohydrolase III from Methanocaldococcus jannaschii Reveals a Two Metal Ion Catalytic …
2008	Low-frequency mode activity of heme: femtosecond coherence spectroscopy of iron porphine halides and nitrophorin
2008	Binding of YC-1 or BAY 41-2272 to soluble guanylyl cyclase induces a geminate phase in CO photolysis
2008	Allostery in recombinant soluble guanylyl cyclase from Manduca sexta
2008	N15 Cro and λ Cro: Orthologous DNA‐binding domains with completely different but equally effective homodimer interfaces
2007	Assignment of the Ferriheme Resonances of the Low-Spin Complexes of Nitrophorins 1 and 4 by 1H and 13C NMR Spectroscopy: Comparison to Structural Data …
2007	Nonequilibrium dynamics simulations of nitric oxide release: comparative study of nitrophorin and myoglobin
2007	Apo‐nitrophorin 4 at atomic resolution
2007	Haem proteins
2007	X-ray Structure of Cerulean GFP: A Tryptophan-Based Chromophore Useful for Fluorescence Lifetime Imaging,
2007	Microsystems for UV-visible and x-ray analysis of protein crystals
2007	S-nitrosylation-induced conformational change in blackfin tuna myoglobin
2007	Buried S-Nitrosocysteine Revealed in Crystal Structures of Human Thioredoxin,
2006	Structural characterization of R. prolixus nitrophorin 4 by UV and visible resonance Raman spectroscopy
2006	Structure of the Y94F mutant of Escherichia coli thymidylate synthase
2006	O19. Crystal structures of S-nitrosated thioredoxin
2006	P062. NO and GSNO stimulation of recombinant soluble guanylate cyclase from Manduca sexta
2006	Crystal structures of thymidylate synthase mutant R166Q: structural basis for the nearly complete loss of catalytic activity
2006	O11. HNO donors and heme proteins
2006	SYMPOSIUM LECTURES-Nitrosyl heme and S-nitrosation in nitric oxide signaling
2005	5, 10-Methylene-5, 6, 7, 8-tetrahydrofolate conformational transitions upon binding to thymidylate synthase: molecular mechanics and continuum solvent studies
2005	Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein
2005	Ultrahigh Resolution Structures of Nitrophorin 4: Heme Distortion in Ferrous CO and NO Complexes,
2005	A Novel Copper-Binding Fold for the Periplasmic Copper Resistance Protein CusF,
2004	Cuprous Oxidase Activity of CueO from Escherichia coli
2004	Axial ligand complexes of the Rhodnius nitrophorins: reduction potentials, binding constants, EPR spectra, and structures of the 4-iodopyrazole and imidazole …
2004	Structural dynamics controls nitric oxide affinity in nitrophorin 4
2004	HEME DEFORMATION AND A NOVEL HEME-ASSISTED NITROSILATION OF A THIOLATE LIGAND IN NITROPHORINS FROM BLOOD-SUCKING INSECTS
2004	Protein Functional Cycle Viewed at Atomic Resolution: Conformational Change and Mobility in Nitrophorin 4 as a Function of pH and NO Binding,
2004	Role of Binding Site Loops in Controlling Nitric Oxide Release: Structure and Kinetics of Mutant Forms of Nitrophorin 4,
2004	Heme ruffling in nitrophorin 4, a NO transport protein from Rhodnius prolixus
2004	Cimex lectularius nitrophorin characterized by resonance Raman spectroscopy
2004	Loop dynamics and function in nitrophorin 4
2004	Resonance Raman spectroscopic study of nitrophorin 2 from Rhodnius prolixus kissing bug and its mutants
2004	Protein and ligand induced heme distortion in the nitrophorins
2003	Complementing thymidylate synthase
2003	Redox potential of human thioredoxin 1 and identification of a second dithiol/disulfide motif
2003	Nitric oxide transport by blood-sucking insects: Structure and dynamics
2003	A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO
2002	Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli
2002	Nitrophorins: Lipocalin‐based heme proteins transporting nitric oxide
2002	Blocking ligand escape with a stochastic gate in nitrophorin-NO complex
2001	Spectroelectrochemical studies of nitrophorin mutants: The importance of the carboxylates near the heme.
2001	INSECT Rhodnius prolixus
2001	Ligand-Induced Heme Ruffling and Bent NO Geometry in Ultra-High-Resolution Structures of Nitrophorin 4,
2001	Properties and biological activities of thioredoxins
2001	Resonance Raman Spectroscopic Study of Nitrophorin 1, a Nitric Oxide-Binding Heme Protein from Rhodnius prolixus, and Its Nitrosyl and Cyano Adducts
2000	Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial
2000	The nitric oxide-releasing heme proteins from the saliva of the blood-sucking insect Rhodnius prolixus
2000	α-Cyclodextrin extracts diacylglycerol from insect high density lipoproteins
2000	Nitrophorins and related antihemostatic lipocalins from Rhodnius prolixus and other blood-sucking arthropods
2000	The crystal structure of Nitrophorin 2: A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus
2000	THE NITRIC OXIDE-RELEASING HEME PROTEINS
2000	Nitric oxide transport by a blood-sucking insect: Nitrophorin structure, dynamics, and distal pocket burial
2000	Kinetics and equilibria in ligand binding by nitrophorins 1− 4: Evidence for stabilization of a nitric oxide− ferriheme complex through a ligand-induced conformational trap
1999	Nitric oxide binding to the ferri-and ferroheme states of nitrophorin 1, a reversible NO-binding heme protein from the saliva of the blood-sucking insect, Rhodnius prolixus
1999	Structural, spectroscopic and electrochemical comparison of three of the four nitrophorins (NO-releasing heme proteins) from the saliva of the blood-sucking insect, Rhodnius …
1999	In vivo inhibition of histamine-induced vascular leaks with kissing bug (R prolixus) salivary protein NP4.
1999	Structure and reactivity of nitrophorins from a blood-sucking insect
1999	Crystal Structures of Rat Thymidylate Synthase Inhibited by Tomudex, a Potent Anticancer Drug,
1998	The crystal structure of nitrophorin 4 at 1.5 Å resolution: transport of nitric oxide by a lipocalin-based heme protein
1998	Crystal structures of a nitric oxide transport protein from a blood-sucking insect
1998	Mode of action of site-directed irreversible folate analogue inhibitors of thymidylate synthase
1997	Human Thioredoxin Homodimers: Regulation by pH, Role of Aspartate 60, and Crystal Structure of the Aspartate 60 → Asparagine Mutant,
1997	Crystal structures of a marginally active thymidylate synthase mutant, Arg 126→ Glu
1997	Nitric Oxide Binding and Crystallization of Recombinant Nitrophorin I, a Nitric Oxide Transport Protein from the Blood-Sucking Bug Rhodnius prolixus
1997	Thymidylate synthase: structure, inhibition, and strained conformations during catalysis
1997	Reversible no-carrying heme proteins from the saliva of bloodsucking insects.
1997	Use of strain in a stereospecific catalytic mechanism: crystal structures of Escherichia coli thymidylate synthase bound to FdUMP and methylenetetrahydrofolate
1996	Oxidative inactivation of thioredoxin as a cellular growth factor and protection by a Cys73→ Ser mutation
1996	Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer
1995	Ligand-induced distortion of an active site in thymidylate synthase upon binding anticancerdrug 1843U89
1995	Promotion of purine nucleotide binding to thymidylate synthase by a potent folate analogue inhibitor, 1843U89.
1995	Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase …
1994	Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex
1990	Pairwise Specificity and Sequential Binding in Enzyme Catalysis: Thymidylate SynthaseT
1990	Pairwise specificity and sequential binding in enzyme catalysis: thymidylate synthase
1990	Synthase
1990	Segmental accommodation: A novel conformational change induced upon ligand binding by thymidylate synthase
1990	Binding by Thymidylate Synthase1
1990	Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases
1990	Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate
1987	Atomic structure of thymidylate synthase: target for rational drug design
1987	The three-dimensional structure of ricin at 2.8 A.
1985	Coordination chemistry of alkali and alkaline earth cations: Synthesis and X-ray crystal structure of cesium (picrate)(benzo-15-crown-5) Cs+ C 6 H 2 N 3 O 7−(C 14 H 20 O 5)
1985	X-ray crystallographic studies on the toxic protein ricin
1984	THE X-RAY STRUCTURE OF RICIN AND IMPLICATIONS FOR IMMUNOTOXIN DESIGN

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