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We have continued and plan to expand the guided inquiry research component of our one semester Biochemistry Laboratory Course (BIOC463A). Since 2009, the second portion of the semester involved a Special Research Project. Initially our studies involved examination of the structural importance and roles of two disulfide bonds in alkaline phosphatase, a periplasmic enzyme from E. coli, using the chemical reducing agents beta-mercaptoethanol, dithiothreitol, and triscarboxyethylphosphine. These early studies included a variety of spectroscopic methods including activity assays, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy.Show Less
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Courses
- BBiochemistry
- FBFoundations in Biochemistry
- BLTBiochemical Laboratory Techniques
Grants
- MRI: Acquisition of a State-of-the-Art Analytical Ultracentrifuge (AUC) For Biomedical and Materials Research
Co-Investigator (COI)
2020
$342.9K
Active - Radiation and Fluorescence Imaging Technology for Biology and Biochemistry
Principal Investigator (PI)
2013
$126.7K
News
- Open Access Week 2012: Raising Awareness at the UA
2012
- UA Researchers Study Use of Peptide Hormones in Treating Disease
2005
Publications (28)
Recent
- The run-on oligomer filament enzyme mechanism of SgrAI. Part 2: Kinetic modeling of the full DNA cleavage pathway
2018
- The run-on oligomer filament enzyme mechanism of SgrAI. Part 1: Assembly kinetics of the run-on oligomer filament
2018
- Rational design of a hexameric protein assembly stabilized by metal chelation
2018
- A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation
2015
- Probing the run-on oligomer of activated SgrAI bound to DNA
2015
- Structure and specificity of FEN-1 from Methanopyrus kandleri
2015
- A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection
2013
- Structural analysis of activated SgrAI--DNA oligomers using ion mobility mass spectrometry
2013
- Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization
2013
- Activation by Oligomerization of an Allosteric Sequence Specific Endonuclease
2012
- Zinc enhances adiponectin oligomerization to octadecamers but decreases the rate of disulfide bond formation
2012
- Dimerization in the Grb7 protein
2012
- Crystallization of zinc finger proteins bound to DNA
2010
- Activation of DNA cleavage by oligomerization of DNA-bound SgrAI
2010
- Domain swapping in allosteric modulation of DNA specificity
2010
- Unique agonist-bound cannabinoid CB 1 receptor conformations indicate agonist specificity in signaling
2008
- Development of novel enkephalin analogues that have enhanced opioid activities at both $mu$ and $delta$ opioid receptors
2007
- Plasmon resonance methods in GPCR signaling and other membrane events
2005
- Adhesion and coalescence of ductile metal surfaces and nanoparticles
2003
- Long-range interaction forces between polymer-supported lipid bilayer membranes
2001
- Formation of tethered supported bilayers by vesicle fusion onto lipopolymer monolayers promoted by osmotic stress
2000
- Polymer-cushioned bilayers. II. An investigation of interaction forces and fusion using the surface forces apparatus
1999
- Polymer-cushioned bilayers. I. A structural study of various preparation methods using neutron reflectometry
1999
- Film balance and fluorescence microscopic investigation of the effects of Ca2+ on mixed DMPC/DMPG monolayers
1999
- Structural studies of polymer-cushioned lipid bilayers
1998
- Direct surface force and contact angle measurements of an adsorbed polymer with a lower critical solution temperature
1998
- Formation of tethered supported bilayers via membrane-inserting reactive lipids
1998
- Structure and Forces in Transfection Related Surfactant Systems
1997