ABSTRACTAmerican Indians and Alaska Natives (AIAN) are less likely to participate in longitudinal studies in clinical trials and in genetic testing and are a hard-to-reach population due to smalloverall population size and a general mistrust of science research and the Federal government. To meet their inclusion goals the All of Us program (AOU) must continue to build trust withTribal Nations. Our proposed project is developed specifically to build trust through strategiesthat honor key values identified by community members and Tribal leaders. One of the commitments NIH/AOU made following the Tribal consultation process is to firstengage with tribal communities through smaller pilot projects with Tribal Nations and urban and rural tribal communities and will use the information gathered to evaluate the successes and failures before scaling up any collaborations with tribes.To help AOU meet this promise the community readiness assessment (CRA) will identifynations that are interested in pilot projects and which need greater support in capacity building todo so. A community readiness survey will be developed and pilot tested. We will work withTribal/Community Advisors to develop and implement the survey(s) and tools and guidance insupporting capacity building efforts for Tribes/communities at lower levels of readiness. We aimto: Establish an Advisory Council of Tribal and urban AIAN community leaders to develop and implement a Community Readiness Assessment (CRA) survey; Provide feedback to support Tribal partners and the AOU research program to prioritizeappropriate strategies for building partnerships; Document best practices in building such partnerships; In collaboration with the AOU Tribal Engagement Office develop a strategic plan for long-term Tribal engagement for the program; and Present the products/materials to the NIH Tribal Advisory Council for endorsement.