PROJECT SUMMARYThis application is for a shared isothermal titration microcalorimeter (ITC) facility. ITC technique enables theaccurate determination of ligand receptor dissociation constants and complete thermodynamic parameterswhich will strengthen the research projects of a group of very productive NIH-supported faculty members inmedicinal chemistry pharmacology & toxicology chemistry & biochemistry molecular biology and biophysicsat the University of Arizona (UA). These investigators are funded by NIH to study the design and synthesis ofsmall molecules for novel therapeutic targets to develop small-molecule probes to perturb biological processesassociated with various diseases or to investigate intermolecular interactions driving biological processes. Theirresearch addresses a wide variety of unmet medical needs including microbial infections neurological disordersdiabetes and cancer. Currently there is no ITC at the University of Arizona with low sample volume andautomated sample handling capabilities. The requested instrument will have a significant impact on a large groupof NIH-supported major and new users particularly for hit validation from high throughput screening andmedicinal chemistry campaigns. Further we are confident that the new ITC will stimulate new collaborationsamong investigators at UA. The Arizona Center for Drug Discovery (ACDD) will house the instrument and provideuser training for the ITC while College of Pharmacy and the Office of Research Innovation and Impact (RII) willsupport for the service contract. Scheduling of the instrument will be through web-based iLab Organizer andany conflicts will be resolved through an advisory committee.