KMap
David Galbraith obtained undergraduate and graduate degrees in Biochemistry from the University of Cambridge, and postdoctoral training as a NATO Fellow at Stanford University. His first academic appointment was at the University of Nebraska Lincoln, and he became Professor of Plant Sciences at the University of Arizona in 1989. His research has focused on the development of instrumentation and methods for the analysis of biological cells, organs, and systems. He is internationally recognized as a pioneer in the development and use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. He also was among the first to develop methods for the analysis of gene expression within specific cell types, using markers based on Fluorescent Protein expression for flow sorting these cells, and microarray platforms for analysis of their transcriptional activities and protein complements. Current interests include applications of highly parallel platforms for transcript and protein profiling of minimal sample sizes, and for analysis of genetic and epigenetic mechanisms that regulate gene expression during normal development and in diseased states, specifically pancreatic cancer. He is also funded to study factors involved in the regulation of bud dormancy in Vitis vinifera, and has interests in biodiversity and improvement of third-world agriculture. He has published more than 180 scholarly research articles, holds several patents, was elected a Fellow of the American Association for Advancement of Science in 2002, and serves on the editorial board of Cytometry Part A. He is widely sought as a speaker, having presented over 360 seminars in academic, industrial and conference settings. He was elected Secretary of the International Society for Advancement of Cytometry in 2016. Keywords: Plant and Animal Cellular Engineering; Biological Instrumentation; Flow Cytometry and Sorting

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Courses
  • MCBG
    Methods in Cell Biology and Genomics

  • PBME
    Plant Biochemistry and Metabolic Engineering

Grants
  • Funding agency logo
    Bench fees for Nadeem Munawar

    Principal Investigator (PI)

    2019

    $3.5K
  • Funding agency logo
    Bench fees for Adnan Iqbal

    Principal Investigator (PI)

    2018

    $3.5K
  • Funding agency logo
    Bench fees for Beenish Afzal

    Principal Investigator (PI)

    2018

    $3.5K
  • Funding agency logo
    Bench fees for Samina Bashir

    Principal Investigator (PI)

    2018

    $3.5K
  • Funding agency logo
    Higher Education Commission Pakistan Scholarship

    Principal Investigator (PI)

    2016

    $2.8K
  • Funding agency logo
    RAPID: Development of a Handheld, Rapid Molecular Diagnostic Tool for Ebola

    Principal Investigator (PI)

    2015

    $211.2K
  • Funding agency logo
    Identification of Changes in Gene Expression at the Earliest Stages of Prostate Oncogenesis

    Principal Investigator (PI)

    2014

    $100.0K
  • Funding agency logo
    Characterization and manipulation of primary components potentially involved in ABA-mediated repression of grape bud dormancy release and in its...

    Principal Investigator (PI)

    2013

    $105.0K
  • Funding agency logo
    Cell-specific analysis of transcription and epigenomic status in PDAC

    Principal Investigator (PI)

    2012

    $350.6K
  • Funding agency logo
    miRNA HD Array Platform (SBIR Project)

    Principal Investigator (PI)

    2009

    $220.1K
News
  • New Look Inside Cell Nucleus Could Improve Cancer Diagnostics

    2013

  • 24 Hours and More Than 30,000 Meals

    2010

  • Student Union Introduces New Restaurant, Revamps U-Mart

    2009

  • Research May Help Plants, Humans Survive Stress, Disease

    2009

  • UA Scientist Studied Nobel Prize-Winning Idea

    2008

  • Highland Market Now Open 24/7

    2008

  • Starbucks Opens in the Union

    2008

  • Plant Scientists Develop New Cell-Sorting Technique

    2008

  • New Health-Conscious Restaurant Opens in Union

    2007

  • Redington Restaurant Now Offers Breakfast

    2006

  • IQ Fresh Restaurant Now Open in Student Union

    2004

  • Highland Market Set to Open

    2004

  • Scientists Map What Makes a Root a Root

    2003

  • Unlocking the Genetic Codes of Maize and Rice

    2003

  • Working Together: Biomedical Science and Biotechnology Institute to Promote Interdisciplinary Research

    2002

  • Year in Review 2002: Incredible Science

    2002

  • Seven UA Scientists Elected AAAS Fellows for 2002

    2002

Publications (194)
Recent
  • Validation of crowd‐sourced plant genome size measurements

    2022

  • Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis

    2022

  • Application-based guidelines for best practices in plant flow cytometry

    2021

  • Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

    2021

  • Shapiro's Laws Revisited: Conventional and Unconventional Cytometry at CYTO2020

    2021

  • Best practices in the flow cytometry of microalgae

    2021

  • Maize single-nucleus transcriptomes comprehensively describe signaling networks governing movement and development of grass stomata.

    2021

  • Flow Cytometry and Sorting in Arabidopsis.

    2021

  • Best practices in plant cytometry

    2021

  • The chromosome-level Thlaspi arvense genome provides new tools for translational research and for a newly domesticated cash cover crop of the cooler climates.

    2021

  • From mouse to mouse-ear cress: nanomaterials as delivery carriers in plant biotechnology.

    2021

  • The use of flow cytometry for estimating genome sizes and DNA ploidy levels in plants.

    2021

  • Validation of crowd-sourced plant genome size measurements

    2021

  • Identification of potential post-ethylene events in the signaling cascade induced by stimuli of bud dormancy release in grapevine

    2020

  • Identification of post-ethylene events in the signaling cascade induced by dormancy release stimuli.

    2020

  • Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.)

    2020

  • Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength

    2020

  • Chemical control of rodents and its impact on rodent infestations during subsequent cropping season

    2020

  • Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

    2019

  • BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize

    2019

  • Optimizing Periplasmic Expression in Escherichia coli for the Production of Recombinant Proteins Tagged with the Small Metal-Binding Protein SmbP

    2019

  • A spatiotemporal DNA endoploidy map of the Arabidopsis root reveals roles for the endocycle in root development and stress adaptation.

    2018

  • Distinct gibberellin functions during and after grapevine bud dormancy release

    2018

  • A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation

    2018

  • Transient induction of a subset of ethylene biosynthesis genes is potentially involved in regulation of grapevine bud dormancy release.

    2018

  • Distinct functions for gibberellin during and after grape bud dormancy release

    2018

  • Nuclear Cytometry: Analysis of the Patterns of DNA Synthesis and Transcription Using Flow Cytometry, Confocal Microscopy, and RNA Sequencing

    2018

  • ABA catabolism enhances dormancy release of grapevine buds.

    2018

  • Abscisic acid catabolism enhances dormancy release of grapevine buds

    2018

  • Mie scatter and interfacial tension based real-time quantification of colloidal emulsion nucleic acid amplification

    2017

  • Guidelines for the use of flow cytometry and cell sorting in immunological studies

    2017

  • Flow cytometry and single nucleus sorting for Cre-based analysis of changes in transcriptional states

    2016

  • Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress.

    2016

  • Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF

    2016

  • Redrawing the frontiers in the age of post-publication review

    2015

  • Digital data acquisition and processing

    2015

  • TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis

    2014

  • Endoreduplicative standards for calibration of flow cytometric C-Value measurements

    2014

  • Challenges and Solutions in Cytometric Measurements of Non-Mammalian Species

    2014

  • Flow cytometry and sorting in Arabidopsis

    2014

  • Genome size in the Fagaceae and its general implications for trees.

    2014

  • RNA-Seq from single nuclei

    2013

  • RNA-sequencing from single nuclei

    2013

  • Identification and characterization of a repertoire of genes differentially expressed in developing top ear shoots between a superior hybrid and its parental inbreds in Zea mays L.

    2013

  • The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads

    2012

  • RNA interference-mediated gene knockdown within specific cell types

    2012

  • Enhanced salt stress tolerance of rice plants expressing a vacuolar H sup + /sup -ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel

    2012

  • Enhanced salt stress tolerance of rice plants expressing a vacuolar H+-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora

    2012

  • Flow cytometry and fluorescence-activated cell sorting in plants: the past, present, and future

    2012

  • High-throughput monitoring of plant nuclear DNA contents via flow cytometry

    2012

  • A high-density quantitative nuclease protection microarray platform for high throughput analysis of gene expression

    2011

  • The grand challenges in enabling data-intensive biological research

    2011

  • Multiparametric analysis, sorting, and transcriptional profiling of plant protoplasts and nuclei according to cell type

    2011

  • Optimizing microarray-based in situ transcription-translation of proteins for matrix-assisted laser desorption ionization mass spectrometry

    2011

  • Dissecting quantitative resistance against blast using heterogeneous inbred families in rice

    2011

  • Barcodes for DNA sequencing with guaranteed error correction capability

    2011

  • The genomes of all angiosperms: a call for a coordinated global census

    2011

  • Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice

    2011

  • Analysis and synthesis of boolean cell-cycle gene regulatory networks

    2011

  • Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry

    2011

  • Frontiers in genomic assay technologies: The grand challenges in enabling data-intensive biological research

    2011

  • Functional analysis of the gossypium arboreum genome

    2010

  • Applications of microarrays for crop improvement: Here, there, and everywhere

    2010

  • Genome structures and halophyte-specific gene expression of the extremophile thellungiella parvula in comparison with Thellungiella salsuginea (Thellungiella halophila) and arabidopsis

    2010

  • Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

    2010

  • Transcript profiling in Vitis riparia during chilling requirement fulfillment reveals coordination of gene expression patterns with optimized bud break

    2009

  • Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis

    2009

  • Measuring genome size of desert plants using dry seeds

    2009

  • Nucleomics: Targeting and Genetic Analysis of Nutrient-Sensing Cells

    2009

  • Analysis of Photoperiod Sensitivity5 sheds light on the role of phytochromes in photoperiodic flowering in rice

    2009

  • Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values

    2009

  • Evidence for antisense transcription associated with microRNA target mRNAs in arabidopsis

    2009

  • RiceArrayNet: A database for correlating gene expression from transcriptome profiling, and its application to the analysis of coexpressed genes in rice

    2009

  • Plant systems biology comes of age

    2008

  • Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice

    2008

  • Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei

    2008

  • Isolation of candidate genes for tolerance of abiotic stresses.

    2008

  • Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors

    2008

  • Persistent infection and promiscuous recombination of multiple genotypes of an RNA virus within a single host generate extensive diversity

    2007

  • Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release

    2007

  • Protoplast Analysis using Flow Cytometry and Sorting

    2007

  • Analysis of Plant Gene Expression Using Flow Cytometry and Sorting

    2007

  • Nucleomics of glucose sensing cells: methodology for cell-type specific analysis of gene expression

    2007

  • High-throughput, high-sensitivity analysis of gene expression in arabidopsis

    2007

  • Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells

    2007

  • Nanobiotechnology: Silica breaks through in plants

    2007

  • Involvement of calcium signalling in dormancy release of grape buds

    2007

  • No DNA loss in autotetraploids of Arabidopsis thaliana

    2006

  • The Oryza bacterial artificial chromosome library resource: Construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza

    2006

  • DNA microarray analyses in higher plants

    2006

  • A framework of integrating gene relations from heterogeneous data sources: an experiment on Arabidopsis thaliana

    2006

  • Development of a comprehensive detection method for medicinal and toxic plant species.

    2006

  • Global studies of cell type-specific gene expression in plants

    2006

  • Meiotically stable natural epialleles of Sadhu, a novel Arabidopsis retroposon.

    2006

  • AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis

    2006

  • The daunting process of MIAME [2]

    2006

  • Cell type-specific characterization of nuclear DNA contents within complex tissues and organs

    2005

  • Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome

    2005

  • Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression

    2005

  • SYMBIODINIUM (PYRRHOPHYTA) GENOME SIZES (DNA CONTENT) ARE SMALLEST AMONG DINOFLAGELLATES

    2005

  • Cell type-specific expression profiting in plants via cell sorting of protoplasts from fluorescent reporter lines

    2005

  • Design, production, and utilization of long oligonucleotide microarrays for expression analysis in maize

    2005

  • Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry

    2005

  • Performance analysis of a dual-buffer architecture for digital flow cytometry

    2005

  • Methods for transcriptional profiling in plants. Be fruitful and replicate

    2004

  • Transcript expression profiles of Arabidopsis thaliana grown under controlled conditions and open-air elevated concentrations of CO sub 2 /sub and of O sub 3 /sub

    2004

  • Integrative flow cytometric and microarray approaches for use in transcriptional profiling.

    2004

  • A probable Na sup + /sup (K sup + /sup )/H sup + /sup exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana

    2004

  • Salt cress. A halophyte and cryophyte arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles

    2004

  • A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana

    2004

  • Cytometry and plant sciences: a personal retrospective

    2004

  • Nuclear dynamics in higher plants

    2004

  • The rainbow of fluorescent proteins

    2004

  • The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy.

    2003

  • The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress

    2003

  • Temporal progression of gene expression responses to salt shock in maize roots

    2003

  • A gene expression map of the Arabidopsis root

    2003

  • Global analysis of cell type-specific gene expression

    2003

  • Factors affecting nuclear dynamics and green fluorescent protein targeting to the nucleus in Arabidopsis thaliana roots

    2002

  • Improved data acquisition system for digital flow cytometry

    2002

  • Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

    2002

  • Comparison of RNA expression profiles based on maize expressed sequence tag frequency analysis and micro-array hybridization

    2002

  • GENOMICS-BASED UNDERSTANDING OF ABIOTIC STRESS RESPONSES IN PLANTS :

    2001

  • Microarray-based analysis of gene expression in very large gene families: the cytochrome P450 gene superfamily of Arabidopsis thaliana

    2001

  • Gene expression profiling of salinity stress responses using expressed sequence tag (EST)-based microarrays in the common ice plant, Mesembryanthemum crystallinum

    2001

  • Modulation of Abscisic Acid Signal Transduction and Biosynthesis by an Sm-like Protein in Arabidopsis

    2001

  • Microarray-based survey of repetitive genomic sequences in Vicia spp.

    2001

  • CYP83B1, a cytochrome P450 at the metabolic branch paint in auxin and indole glucosinolate biosynthesis in Arabidopsis

    2001

  • Gene expression profiles during the initial phase of salt stress in rice

    2001

  • A genomics approach towards salt stress tolerance

    2001

  • Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba

    2001

  • High-density microarrays for gene expression analysis

    2001

  • Analysis of nuclear DNA content and ploidy in higher plants.

    2001

  • Printing DNA microarrays using the Biomek 2000 laboratory automation workstation.

    2001

  • Nuclear dynamics in Arabidopsis thaliana

    2000

  • Implementing microarrays for the study of plant genomics

    1999

  • Flow cytometric analysis and FACS sorting of cells based on GFP accumulation

    1999

  • Insect-induced synthesis of phytoecdysteroids in spinach, Spinacia oleracea

    1999

  • Reference standards for determination of DNA content of plant nuclei

    1999

  • Green fluorescent protein targeted to the nucleus, a transgenic phenotype useful for studies in plant biology

    1999

  • Flow cytometric analysis of transgene expression in higher plants: Green fluorescent protein

    1999

  • Adapting the Biomek® 2000 Laboratory Automation Workstation for printing DNA microarrays

    1998

  • Isolation and characterization of an Arabidopsis thaliana C-8,7 sterol isomerase: Functional and structural similarities to mammalian C-8,7 sterol isomerase/emopamil-binding protein

    1998

  • Nuclear expressed sequence Tag (NEST) analysis: a novel means to study transcription through amplification of nuclear RNA

    1998

  • Damage-induced accumulation of phytoecdysteroids in spinach: A rapid root response involving the octadecanoic acid pathway

    1998

  • Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis - Isolation of mutations in the cytochrome P450 gene superfamily

    1998

  • Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants

    1997

  • Green-fluorescent protein fusions for efficient characterization of nuclear targeting

    1997

  • Studies on the mode of action of cholesterol oxidase on insect midgut membranes

    1997

  • Characterization of Zea mays endosperm C-24 sterol methyltransferase: One of two types of sterol methyltransferase in higher plants

    1997

  • Systematic reverse genetics of the P450 gene family in arabidopsis thaliana

    1997

  • Automated particle classification based on digital acquisition and analysis of flow cytometric pulse waveforms

    1996

  • Ecdysone 20-monooxygenase, a cytochrome P450 enzyme from spinach, Spinacia oleracea

    1996

  • Z-membranes: Artificial organelles for overexpressing recombinant integral membrane proteins

    1996

  • Preface

    1995

  • Neural network analysis of digital flow cytometric data

    1995

  • Green-fluorescent protein as a new vital marker in plant cells

    1995

  • Flow cytometric analysis using digital signal processing

    1995

  • GREEN-FLUORESCENT PROTEIN AS A NEW VITAL MARKER IN PLANT-CELLS

    1995

  • Chapter 1 Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green-Fluorescent Protein

    1995

  • The callus associated protein (CAP) gene of Nicotiana tabacum: Isolation, characterization, and evidence for possible function as a transcriptional factor

    1994

  • Chapter 31 Flow Cytometry and Sorting of Plant Protoplasts and Cells

    1994

  • Flow cytometry and sorting of plant protoplasts and cells

    1994

  • Biosynthesis, processing and targeting of the G-protein of vesicular stomatitis virus in tobacco protoplasts

    1992

  • SYSTEMIC ENDOPOLYPLOIDY IN ARABIDOPSIS-THALIANA

    1991

  • Systemic endopolyploidy in Arabidopsis thaliana

    1991

  • Flow cytometric analysis of plant genomes

    1990

  • Systemic endopolyploidy in Arabidopsis thaliana.

    1990

  • Developmentally regulated systemic endopolyploidy in succulents with small genomes

    1990

  • Isolation and flow cytometric characterization of plant protoplasts

    1990

  • Chapter 48 Flow Cytometric Analysis of Plant Genomes

    1990

  • Chapter 47 Isolation and Flow Cytometric Characterization of Plant Protoplasts

    1990

  • Analysis of Higher Plants by Flow Cytometry and Cell Sorting

    1989

  • Flow cytometric characterization of the chlorophyll contents and size distributions of plant protoplasts.

    1988

  • Isolation and characterization of monoclonal antibodies directed against plant plasma membrane and cell wall epitopes: Identification of a monoclonal antibody that recognizes extensin and analysis of the process of epitope biosynthesis in plant tissues and cell cultures

    1988

  • Storage protein accumulation patterns in somatic embryos of cotton (Gossypium hirsutum L.)

    1987

  • Factors governing the flow cytometric analysis and sorting of large biological particles

    1987

  • Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)

    1986

  • Selection of Somatic Hybrid Plants in Nicotiana Through Fluorescence-Activated Sorting of Protoplasts

    1985

  • Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana

    1984

  • Presence of a plant cell wall is not required for transformation of Nicotiana by Agrobacterium tumefaciens

    1984

  • Flow sorting and culture of plant protoplasts

    1984

  • Rapid flow cytometric analysis of the cell cycle in intact plant tissues

    1983

  • Improvement of Anther Culture in Nicotiana: Media, Cultural Conditions and Flow Cytometric Determination of Ploidy Levels

    1983

  • The effects of inhibitors of cell wall synthesis on tobacco protoplast development

    1982

  • Microfluorimetric quantitation of cellulose biosynthesis by plant protoplasts using Calcofluor White

    1981

  • Analysis of the initial stages of plant protoplast development using 33258 Hoechst: reactivation of the cell cycle

    1981

  • The isolation of plasma membrane from protoplasts of soybean suspension cultures

    1977

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