KMap
The assembly of viruses inside infected cells involves numerous protein-protein interactions. These interactions are often orchestrated by scaffolding proteins. Analogous to scaffoldings used in building construction, these proteins ensure the integrity and efficiency of progeny formation but are not found in the final product. Once the virus is assembled, it must transport its genetic material to the next cell to be infected. The broad objective of our research program is to elucidate the molecular mechanisms involved in scaffolding-mediated assembly and the transportation of the viral genome through a specialized DNA-translocating conduit.

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Courses
  • MP
    Microbial Physiology

Grants
  • Funding agency logo
    Icosahedral Virion Assembly and Function

    Principal Investigator (PI)

    2020

    $799.2K
    Active
  • Funding agency logo
    Icosahedral Virion Assembly and Function

    Principal Investigator (PI)

    2015

    $1.0M
  • Funding agency logo
    Icosahedral Virion Assembly

    Principal Investigator (PI)

    2010

    $981.0K
Books
  • Microviridae: Microviruses and Gokushoviruses

    2011

News
  • The Value of Nutrition and Exercise, According to a Moth

    2017

  • The Virus That Makes a Disposable Syringe

    2013

  • Two UBRP Mentors Earn Accolades

    2013

  • Centennial Award Winners Announced

    2009

  • UA Researchers Engineer Self-Destructing Virus

    2008

Publications (68)
Recent
  • Cryo-EM Structure of Gokushovirus ΦEC6098 Reveals a Novel Capsid Architecture for a Single-Scaffolding Protein, Microvirus Assembly System

    2022

  • Low temperature adaptation targets genome packing reactions in an icosahedral single-stranded DNA virus

    2022

  • The Effects of Packaged, but Misguided, Single-Stranded DNA Genomes Are Transmitted to the Outer Surface of the ϕX174 Capsid

    2021

  • Finally, a role befitting A star: the strongly conserved, unessential microvirus A* (A star) proteins ensure the product fidelity of packaging reactions

    2020

  • Mutagenic Analysis of a DNA Translocating Tube's Interior Surface

    2020

  • Recessive host range mutants and unsusceptible cells that inactivate virions without genome penetration: ecological and technical implications

    2019

  • Coat Protein Mutations That Alter the Flux of Morphogenetic Intermediates through the ϕX174 Early Assembly Pathway

    2017

  • ϕX174 Procapsid Assembly: Effects of an Inhibitory External Scaffolding Protein and Resistant Coat Proteins In Vitro

    2017

  • Elevating fitness after a horizontal gene exchange in bacteriophage φX174

    2017

  • Hawkmoths use nectar sugar to reduce oxidative damage from flight

    2017

  • Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls

    2017

  • Structure-Function Analysis of the ϕX174 DNA-Piloting Protein Using Length-Altering Mutations

    2016

  • The microviridae: Diversity, assembly, and experimental evolution

    2016

  • PhiXing-it, displaying foreign peptides on bacteriophage ΦX174

    2016

  • The Kinetic and Thermodynamic Aftermath of Horizontal Gene Transfer Governs Evolutionary Recovery

    2015

  • Mutations in the N-terminus of the phiX174 DNA pilot protein H confer both assembly and host cell attachment defects.

    2014

  • Icosahedral phiX174 forms a tail for DNA transport.

    2014

  • High-resolution structure of a virally encoded DNA-translocating conduit and the mechanism of DNA penetration

    2014

  • The evolution of genes within genes and the control of DNA replication in Microviruses

    2014

  • Effects of an early conformational switch defect during φX174 morphogenesis are belatedly manifested late in the assembly pathway

    2013

  • Building the machines: Scaffolding protein functions during bacteriophage morphogenesis

    2012

  • Conformational switch-defective X174 internal scaffolding proteins kinetically trap assembly intermediates before procapsid formation

    2012

  • From resistance to stimulation: the evolution of a virus in the presence of a dominant lethal inhibitory scaffolding protein

    2011

  • In vitro assembly of the øx174 procapsid from external scaffolding protein oligomers and early pentameric assembly intermediates

    2011

  • Uncoupling the functions of a multifunctional protein: The isolation of a DNA pilot protein mutant that affects particle morphogenesis

    2011

  • Viral adaptation to an antiviral protein enhances the fitness level to above that of the uninhibited wild type

    2009

  • N-terminal deletions of the phiX174 external scaffolding protein affect the timing and fidelity of assembly

    2009

  • The expression of N-terminal deletion DNA pilot proteins inhibits the early stages of phiX174 replication

    2009

  • Complete virion assembly with scaffolding proteins altered in the ability to perform a critical conformational switch

    2009

  • Scaffolding proteins altered in the ability to perform a conformational switch confer dominant lethal assembly defects

    2008

  • Behind the chlamydial cloak: The replication cycle of chlamydiaphage Chp2, revealed

    2008

  • Intracellular parasitism of chlamydiae: Specific infectivity of chlamydiaphage Chp2 in Chlamydophila abortus

    2007

  • Characterization and function of putative substrate specificity domain in microvirus external scaffolding proteins

    2007

  • Eliminating the requirement of an essential gene product in an already very small virus: scaffolding protein B-free øX174, B-free

    2007

  • A four-dimensional structure of T4 infection

    2005

  • Identification of an interacting coat-external scaffolding protein domain required for both the initiation of φX174 procapsid morphogenesis and the completion of DNA packaging

    2005

  • The φX174 protein J mediates DNA packaging and viral attachment to host cells

    2004

  • The functions of the N terminus of the phiX174 internal scaffolding protein, a protein encoded in an overlapping reading frame in a two scaffolding protein system

    2004

  • Isolation, molecular characterisation and genome sequence of a bacteriophage (Chp3) from Chlamydophila pecorum

    2004

  • Chlamydiaphage Chp2, a skeleton in the φX174 closet: Scaffolding protein and procapsid identification

    2004

  • Conformational switching by the scaffolding protein D directs the assembly of bacteriophage φX174

    2004

  • Genetic and functional analyses of the øX174 DNA binding protein: the effects of substitutions for amino acid residues that spatially organize the two DNA binding domains

    2004

  • Genetic analyses of putative conformation switching and cross-species inhibitory domains in Microviridae external scaffolding proteins

    2003

  • Host Range of Chlamydiaphages φCPAR39 and Chp3

    2003

  • Structural studies of bacteriophage α3 assembly

    2003

  • Mechanism of scaffolding-assisted viral assembly

    2003

  • Characterization of mutant spectra generated by a forward mutational assay for gene A of ΦX174 from ENU-treated transgenic mouse embryonic cell line PX-2

    2002

  • Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus

    2002

  • phi X174 genome-capsid interactions influence the biophysical properties of the virion: evidence for a scaffolding-like function for the genome during the final stages of morphogenesis

    2002

  • Microviridae, a family divided: Isolation, characterization, and genome sequence of φMH2K, a bacteriophage of the obligate intracellular parasitic bacterium Bdellovibrio bacteriovorus

    2002

  • Molecular characterization of a bacteriophage (Chp2) from Chlamydia psittaci

    2000

  • Efficient complementation by chimeric Microviridae internal scaffolding proteins is a function of the COOH-terminus of the encoded protein

    2000

  • Foreign and chimeric external scaffolding proteins as inhibitors of Microviridae morphogenesis

    2000

  • Cross-functional analysis of the Microviridae internal scaffolding protein

    1999

  • The role of scaffolding proteins in the assembly of the small, single-stranded DNA virus φX174

    1999

  • Structure of a viral procapsid with molecular scaffolding

    1997

  • Genetic analysis of the Φ174 DNA binding protein

    1997

  • Atomic structure of the degraded procapsid particle of the bacteriophage G4: Induced structural changes in the presence of calcium ions and functional implications

    1996

  • Host and φX 174 mutations affecting the morphogenesis or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate

    1995

  • Characterization of the morphogenetic defects conferred by cold-sensitive prohead accessory and scaffolding proteins of φX174

    1995

  • Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage φX174

    1993

  • Functional relationship between the J proteins of bacteriophages φX174 and G4 during phage morphogenesis

    1992

  • Proteolysis of bacteriophage φX174 prohead accessory protein gpB by Escherichia coli OmpT protease is not essential for phage maturation in vivo

    1992

  • Intragenic suppressors of folding defects in the P22 tailspike protein

    1991

  • Identification of global suppressors for temperature-sensitive folding mutations of the P22 tailspike protein

    1991

  • Global suppression of protein folding defects and inclusion body formation

    1991

  • Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage ΦX174

    1991

  • Identification of sites influencing the folding and subunit assembly of the P22 tailspike polypeptide chain using nonsense mutations.

    1987

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